Part one

1. Basic situation of the project

1. Project title: Intelligent analysis of colorectal cancer evolution: Single cell ecological characterization and clinical application based on digital pathology images

2. Project number: JFLKYXM202303AZ-102

3. Project location: Jinfeng Laboratory

4. Procurement method: inquiry

2. Procurement content

1. Construction of colorectal cancer tumor model

• Model A (immune deficiency): A subcutaneous tumor model of human colorectal cancer (SW480 cell line) was constructed on the background of nude mice.

• Model B (immunocompetent): A mouse colorectal cancer (MC38 cell line) tumor model was constructed on the background of C57BL/6J mice.

• Work content: Cell line expansion, animal purchase, vaccination, tumor curve recording.

2. Multicolor flow cytometric analysis (FCM) of tumor microenvironment

• Tissue processing: Tumor tissue needs to be efficiently dissociated to ensure cell viability.

• Detection target: The following groups need to be accurately distinguished through multi-color markers (Panel design must include CD45, CD11b, Ly6G, F4/80, FAP, HOPX, CD3, CD8, CD4, TCF1, PD-1, etc.):

• Neutrophils, macrophages, HOPX+ fibroblasts.

• CD4+ T cells, CD8+ T cells and exhausted precursor CD8+ T cells (Tpex).

• Delivery: Original .fcs file and professional gate strategy analysis report.

3. Liquid chromatography mass spectrometry (LC-MS)

• Sample scope: serum of tumor-bearing mice and serum of clinical patients provided by Party A.

• Testing requirements: Conduct non-targeted or specific metabolic index testing (specific indicators must comply with the research direction of tumor immunometabolism).

• Delivery: Metabolite identification list, differential metabolite heat map, pathway analysis report.

4. Seahorse Mitochondrial Stress Test (Cell Mito Stress Test)

• Detection objects: Tpex cells and HOPX+ fibroblasts after tumor tissue sorting.

• Core indicators: Basal Respiration, ATP production, Maximal Respiration and non-mitochondrial oxygen consumption.

• Technical requirements: It is necessary to solve the technical connection problem between micro-cell sorting and on-machine operation.

3. Business requirements

1. Service cycle: Tumor model construction and delivery: Within 3 months after contract signing and startup.

2. FCM, LC-MS analysis and Seahorse mitochondrial stress test: completed within 120 days after the model meets experimental requirements.

3. Intellectual property rights: All experimental data, in vivo models, technical achievements and related intellectual property rights generated by this project are owned by Party A (Jinfeng Laboratory).

4. Project requirements

1. Qualification requirements: The bidder is registered in the People's Republic of China and has independent legal personality.

2. Technical capabilities: Have mature CRISPR/Cas9 gene editing platform and multi-color immunofluorescence (supporting at least 6 colors and more simultaneous staining) technology reserves.

5. Supplier qualification requirements

(1) Meet the qualifications of Article 22 of the "Government Procurement Law of the People's Republic of China":

1. Have the ability to independently bear civil liability；

2. Have good business reputation and sound financial accounting system；

3. Have the necessary equipment and professional technical capabilities to perform the contract；

4. Have a good record of paying taxes and social security funds in accordance with the law；

5. In the three years before participating in government procurement activities, there are no major illegal records in business activities.；

6. Other conditions stipulated by laws and administrative regulations.

Note: Paragraph 1 requires a business license, organization code certificate and tax registration certificate or public institution legal person certificate. If the three certificates are combined into one, only a business license is required.； Paragraphs 2-6 provide a declaration (see declaration for details).

6. Inquiry schedule

1. Inquiry release time: 10:00 on May 18, 2026

2. Inquiry deadline: 17:00 on May 20, 2026

Suppliers should send the quotation list and relevant attachments (which must be stamped with the official seal and scanned in color) to the contact email 1360191768@qq.com before the inquiry deadline. Those who do not quote before the inquiry deadline will be deemed to have automatically given up.

Supplier quotation documents should include the following attachments (see Part 2 for details):

(1) Quotation letter；

(2) Quotation list；

(3) Legal representative qualification certificate (if the bidder is an authorized representative, he must also provide a legal representative authorization letter)；

(4) Copy of business license or other organization certification documents；

(5) Statement；

(6) Letter of commitment；

(7) Others.

7. Requirements for signature and sealing of quotation documents

1. The bidder should prepare a freshly stamped quotation document (PDF) and send it to the email address. The quotation document must be clearly visible, otherwise the quotation will be deemed invalid.

2. The quotation document should be produced in accordance with the requirements of the "Inquiry Announcement". The quotation document must be signed and sealed by the legal representative of the bidder or his authorized representative at the prescribed signature place. The signature, seal and content should be complete. If there are any omissions, you will bear the risk of being regarded as an invalid quotation.；

3. The quotation document should be written clearly and neatly. Any insertions, alterations, additions or deletions between the lines must be signed or stamped with a personal seal by the legal representative of the bidder or his authorized representative. Quotation documents that are illegible, unclearly expressed, or may lead to non-unique understanding will be deemed invalid.

Part 2.docx