In order to meet the needs of laboratory scientific research work, the biomedical imaging platform will carry out operational skills training on flow cytometry analyzers and laser confocal microscopes. Those who pass the training and assessment will be granted independent operating qualifications for corresponding instruments. Qualified persons can make reservations for use during the opening hours of the instrument. Users who have needs are welcome to sign up.
Training 1: Flow Cytometry Analyzer Operation Training
training time
January 12, 2026 (Monday)
9:30-11:30 am (practical training)
2:00-4:00 pm (assessment)
training location
Room 311, Building 5, Jinfeng Laboratory.
Training content
Basic principles of flow analysis, power on and off and instrument maintenance, DIVA Software use, usage precautions, etc.
Instrument introduction
Instrument name：Flow cytometer
Brand model：BD Fortessa
usage pattern：Make an appointment on time； Entrust an appointment
Instrument introduction：Flow cytometer It is a functional device that performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis of single row cells or biological particles in a fast linear flow state. It is mainly composed of three parts: a liquid flow system, an optical system and an electronic system. It can qualitatively or quantitatively analyze cell proliferation, apoptosis, cytokines, chemokines, fluorescent proteins, antigen-specific responses, cellular immune phenotypes, etc., and is widely used in immunology, molecular biology, oncology, hematology, stem cells and other fields.
Main parameters：
1． Equipped with 5 lasers: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm, up to 18 color analysis and 20 parameter detection can be performed simultaneously；
Registration method
Scan the mini program code to fill in the registration information
Things to note
1． for Assure Training effect, the upper limit of the number of people in this training is10people. Users who have recent usage needs will be given priority to register, and they will need to complete a practical assessment on the afternoon of the training day.
2．After successful registration, you must attend the training on time. If you are indeed unable to participate due to special reasons, you must contact the instrument administrator at least 1 working day in advance to explain the situation and cancel the application. Those who are absent without excuse will be disqualified from training registration within one month.
Training 2: Laser confocal microscope operation training
training time
January 13, 2026 (Tuesday)
9:30-11:30 am
training location
Room 318, Building 3, Jinfeng Laboratory
Training content
Instrument operation steps, usage precautions, etc.
Instrument introduction
Instrument name：confocal laser microscopy
Brand model：ZEISS LSM980
usage pattern：Make an appointment on time； Entrust an appointment
Device functions：This equipment is used to obtain high-definition, high-resolution confocal fluorescence images. Its main functions include: finely observing the morphological changes and internal microstructure of tissues or cells at the cellular and subcellular levels.； Perform tomography on samples to reconstruct and analyze the three-dimensional structure of tissues or cells. ； As well as monitoring dynamic changes in important ion concentrations.
Main accessories:
1．Apochromatic objective: Plan apochromatic dry lens 5×, NA: 0.16； Plan apochromatic dry mirror 10×, NA: 0.45 ； Plan apochromatic dry mirror 20×, NA: 0.8 ； Plan apochromatic dry mirror 40×, NA: 0.95 ； Plan apochromatic oil lens 63×, NA: 1.4 ；
2．4 all-solid-state lasers: 405 nm； 488nm ； 561 nm ； 639 nm ；
3. Detector: 5 physical detectors, including 1 transmitted light DIC detector PMT； 4 confocal fluorescence detectors, including 32-area array GaAsP ultra-high sensitivity detector.
Main parameters：
1．Confocal scanning speed: 13 frames/second (512×512, 16 bits)；
Registration method
Scan the mini program code to fill in the registration information
Things to note
1． In order to ensure the training effect, the upper limit of the number of people in this training is12people. Users who need to use it in the near future will be given priority to register.
2．After successful registration, you must attend the training on time. If you are indeed unable to participate due to special reasons, you must contact the instrument administrator at least 1 working day in advance to explain the situation and cancel the application. Those who are absent without excuse will be disqualified from training registration within one month.
Everyone is welcome to actively participate
Number
023-68059937
Email：Jinfeng@jflab.ac.cn
Address:
No.313, Bella Road Chongqing High-tech Zone
Postal code: 401329
渝公网安备50009802002274