In order to meet the needs of laboratory scientific research work, the biomedical imaging platform will carry out practical training and assessment on flow cytometry analyzers. Those who pass the training and assessment will be granted independent operating rights for the biomedical imaging platform flow cytometer analyzer (Fortessa). Users who have obtained the rights can make reservations for use during the opening hours of the instrument. Users who have needs are welcome to sign up.

Training arrangements

training time

October 23, 2025 (Thursday)

9:30-11:30 am (practical training)

2:00-4:00 pm (assessment)

training location

Room 311, Building 5, Jinfeng Laboratory

Training content

Basic principles of flow analysis, power on/off and instrument maintenance, use of DIVA software, and precautions for use.

Instrument introduction

Instrument name: Flow cytometer

Brand model: BD Fortessa

Usage mode: Make an appointment on time； Entrust an appointment

Instrument introduction: This equipment is a functional equipment that performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis of single-row cells or biological particles in a fast linear flow state. It is mainly composed of three parts: a liquid flow system, an optical system and an electronic system. It can qualitatively or quantitatively analyze cell proliferation, apoptosis, cytokines, chemokines, fluorescent proteins, antigen-specific responses, cellular immune phenotypes, etc., and is widely used in immunology, molecular biology, oncology, hematology, stem cells and other fields.

Main parameters:

1． Equipped with 5 lasers: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm, up to 18 color analysis and 20 parameter detection can be performed simultaneously；

2. Fluorescence sensitivity: FITC < 80 MESF； PE < 30 MESF ；  PE-Cy5 < 10 MESF ； APC < 70 MESF ；

3． Maximum analysis speed: 40000 events/s；

4. Detection resolution: CV < 3%；

5. Detection sensitivity: ≥0.5 μm.

Registration method

Scan the mini program code to fill in the registration information

Things to note

1． In order to ensure the training effect, the maximum number of people in this training is 10 people. Users who have recent usage needs will be given priority to register, and they will need to complete a practical assessment on the afternoon of the training day.

2. After successful registration, you must attend the training on time. If you are indeed unable to participate due to special reasons, you must contact the instrument administrator at least 1 working day in advance to explain the situation and cancel the application. If you are absent without reason, your training registration qualification within one month will be cancelled.

3． During the use of the instrument, you must strictly abide by the "Flow Cytometry Platform Management Rules" and consciously abide by the regulations of the platform.

Everyone is welcome to actively participate