In order to meet the needs of laboratory research, the biomedical imaging platform will carry out practical training and assessment of flow cytometry analyzers. Those who have been trained and passed the assessment will be granted independent operation permissions to the biomedical imaging platform Flow Cytometry Analyzer (Fortessa). Users who have obtained the permission can make an appointment for use during the opening period of the instrument. Users who need to use are welcome to sign up.

Training arrangements

Training time

September 11, 2025

9:30-11:30 am (practical training)

14:00-16:00 pm (assessment)

Training location

Room 311, Building 5, Jinfeng Laboratory

Training content

Basic principles of flow analysis, power switch and instrument maintenance, DIVA software use, precautions, etc.

Instrument Introduction

Instrument name: Flow cytometry analyzer

Brand model: BD Fortessa

Usage mode: Make an appointment on time, entrust an appointment

Instrument introduction: This device is a functional device that performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis of single-row cells or biological particles in a fast linear flow state. It mainly consists of three parts: a liquid flow system, an optical system and an electronic system. Cell proliferation, apoptosis, cytokines, chemokines, fluorescent proteins, antigen-specific reactions, cellular immunophenotypes, etc. can be qualitatively or quantitatively analyzed, and is widely used in fields such as immunology, molecular biology, oncology, hematology, stem cells, etc.

Main parameters:

1. Configure 5 lasers: 355 nm, 405 nm, 488 nm, 561 nm, 640 nm, up to 18 color analysis and 20 parameter detection can be performed simultaneously；

2. Fluorescence sensitivity: FITC < 80 MESF； PE < 30 MESF ；  PE-Cy5 < 10 MESF ； APC < 70 MESF ；

3. Maximum analysis speed: 40000 events/s；

4. Detection resolution: CV < 3%；

5. Detection sensitivity: ≥0.5 μm.

Registration method

Scan the mini program code to fill in the registration information

Things to note

1. To ensure the training results, the maximum number of people training this time is 10. Users who have recently used their needs will be given priority to register, and the actual assessment must be completed on the afternoon of the training.

2. After successful registration, you must participate in the training on time. If you are unable to participate due to special reasons, you must contact the instrument administrator in advance to explain the situation and cancel the application. Those who are absent without reason will not be eligible for training registration within one month.

3. During the use of the instrument, the "Flow Cell Platform Management Rules" must be strictly followed and consciously abide by the various platform regulations.

Everyone is welcome to actively participate