To meet the needs of laboratory research, the biomedical imaging platform will carry out operation skills training for flow cytometry analyzers and laser confocal microscopy. Those who have been trained and passed the assessment will be awarded the independent operating qualifications for the corresponding instrument. Those with the qualifications can make an appointment for use during the opening period of the instrument.

Training 1: Flow Cytometry Analyzer

Training time

August 11, 2025 9:30-11:30

Training location

Room 311, Building 5, Jinfeng Laboratory

Training content

Basic principles of flow analysis, power switch and instrument maintenance, DIVA software use, precautions, etc.

Instrument Introduction

Instrument name: Flow cytometry analyzer

Brand model: BD Fortessa

Usage mode: Make an appointment on time, entrust an appointment

Instrument introduction: This device is a functional device that performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis of single-row cells or biological particles in a fast linear flow state. It mainly consists of three parts: a liquid flow system, an optical system and an electronic system. Cell proliferation, apoptosis, cytokines, chemokines, fluorescent proteins, antigen-specific reactions, cellular immunophenotypes, etc. can be qualitatively or quantitatively analyzed, and is widely used in fields such as immunology, molecular biology, oncology, hematology, stem cells, etc.

Main parameters:

1. 5 lasers are configured: 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm. Up to 18 color analysis and 20 parameter detection can be performed simultaneously;

2. Fluorescence sensitivity: FITC < 80 MESF； PE < 30 MESF ； PE-Cy5 < 10 MESF ； APC < 70 MESF ；

3. Maximum analysis speed: 40000 events/s;

4. Detection resolution: CV < 3%;

5. Detection sensitivity: ≥0.5 μm

Registration method

Scan the mini program code to fill in the registration information

Things to note

1. To ensure the training results, the maximum number of people training this time is 10. Users who have recently used their needs will be given priority to register.

2. After successful registration, you must participate in the training on time. If you are unable to participate due to special reasons, you must contact the instrument administrator in advance to explain the situation and cancel the application. Those who are absent without reason will not be eligible for training registration within one month.

3. During the use of the instrument, the "Flow Cell Platform Management Rules" must be strictly followed and consciously abide by the various platform regulations.

Training 2: Laser confocal microscope

Training time

August 13, 2025 9:30-11:30

Training location

Room 318, Building 3, Jinfeng Laboratory

Training content

Instrument operation steps, precautions, etc.

Instrument Introduction

Instrument name: laser confocal microscope

Brand model: ZEISS LSM980

Usage mode: Make an appointment on time, entrust an appointment

Equipment features: This device is used to obtain high-definition, high-resolution confocal fluorescence images, including careful observation of morphological changes and internal microstructure of tissues or cells at the cell and subcellular levels.； Tomography on the sample can be performed to realize the reconstruction and analysis of the three-dimensional spatial structure of tissue or cell ； And monitor the dynamic changes in the concentration of important ions, etc.

Main accessories:

1. Achromatic objective: flat field achromatic dry mirror 5×, NA: 0.16； Flat field achromatic dry mirror 10×, NA: 0.45 ； Flat field achromatic dry mirror 20×, NA: 0.8 ； Flat field achromatic dry mirror 40×, NA: 0.95 ； Flat field achromatic oil mirror 63×, NA: 1.4 ；

2. All solid laser 4: 405 nm； 488 nm ； 561 nm ； 639 nm ；   

3. Detectors: 5 physical detectors, of which 1 transmit light DIC detector PMT； There are 4 confocal fluorescence detectors, including 32-sided array GaAsP ultra-high sensitivity detector.

Main parameters:

1. Confocal scanning speed: 13 frames/second (512×512, 16 bits);

2. Confocal imaging scanning resolution: free adjustment from 32×1 to 8192×8192 pixels;

3. Scan magnification: The minimum magnification is 0.6×, and 360-degree free rotation scanning can be performed under all scanning methods;

4. Ultra-high resolution imaging resolution: XY direction (transverse) resolution 90nm, Z axis direction (axial) resolution 270nm;

5. Ultra-high resolution imaging speed: 25 frames/second (when scanning resolution is 512×512 pixels).

Registration method

Scan the mini program code to fill in the registration information

Things to note

1. To ensure the training results, the maximum number of people training this time is 12. Users who have recently used their needs will be given priority to register.

2. After successful registration, you must participate in the training on time. If you are unable to participate due to special reasons, you must contact the instrument administrator in advance to explain the situation and cancel the application. Those who are absent without reason will not be eligible for training registration within one month.

3. During the use of the instrument, the "Laser Confocal Imaging Platform Management Rules" must be strictly abide by and consciously abide by the various regulations of the platform.

Everyone is welcome to actively participate