training time

august 6, 2025 9:30-11:30

training location

room 318, building 3, jinfeng laboratory

in order to meet the scientific research needs of the laboratory, the biomedical imaging platform will hold practical training for laser confocal microscopy, including instrument operation steps, usage precautions, etc. those who have passed the training and assessment will be granted independent operation permissions for laser confocal microscopy of the biomedical imaging platform. users who have obtained permission can make an appointment and use it independently during the instrument opening reservation period.

instrument introduction

instrument name: laser confocal microscope

brand model: zeiss lsm980

usage mode: make an appointment on time, entrust an appointment

equipment functions: this equipment is used to obtain high-definition and high-resolution confocal fluorescence images, including carefully observing the morphological changes and internal microstructure of tissue or cell at the cell and subcellular levels; performing tomography on the sample to realize the reconstruction and analysis of the three-dimensional spatial structure of tissue or cell; and monitoring the dynamic changes of important ion concentrations, etc.

main accessories:

1. apochromatic objective: 5× flat field apochromatic drying mirror, na: 0.16; 10× flat field apochromatic drying mirror, na: 0.45; 20× flat field apochromatic drying mirror, na: 0.8; 40× flat field apochromatic drying mirror, na: 0.95; 63× flat field apochromatic oil mirror, na: 1.4;

2. all solid laser 4 strips: 405 nm; 488 nm; 561 nm; 639 nm;

3. detectors: 5 physical detectors, including 1 transmitting dic detector pmt; 4 confocal fluorescence detectors, including 32-sided array gaasp ultra-high sensitivity detector.

main parameters:

1. confocal scanning speed: 13 frames/second (512×512, 16 bits);

2. confocal imaging scanning resolution: free adjustment from 32×1 to 8192×8192 pixels;

3. scan magnification: the minimum magnification is 0.6×, and 360-degree free rotation scanning can be performed under all scanning methods;

4. ultra-high resolution imaging resolution: xy direction (transverse) resolution 90nm, z axis direction (axial) resolution 270nm;

5. ultra-high resolution imaging speed: 25 frames/second (when scanning resolution is 512×512 pixels).

registration method

scan the mini program code below to fill in the information registration

notes:

1. to ensure the training results, the maximum number of people training this time is 6. users who have recently used their needs will be given priority to register.

2. after successful registration, you must participate in the training on time. if you are unable to participate due to special reasons, you must contact the instrument administrator in advance to explain the situation and cancel the application. those who are absent without reason will not be eligible for training registration within one month.

3. during the use of the instrument, the "laser confocal imaging platform management rules" must be strictly abide by and consciously abide by the various regulations of the platform.

everyone is welcome to actively participate